Title: Effect of various chemical solutions on micropropagation efficiency, antioxidant activities and clonal fidelity analysis of in vitro developed plantlets in pomegranate (Punica granatum L.) using SSR marker
Articl link: https://link.springer.com/articl ... -024-00846-y#citeas
Abstract: This study aimed to develop an effective in vitro protocol for micropropagation of pomegranate (Punica granatum L.) var. Bhagwa using nodal explants. To induce shoot growth and cultivating the explants, Murashige and SkoogĄ¯s media were supplemented with benzyl amino purine (BAP), sodium nitroprusside (SNP), an approach demonstrated and experimented for the first time on Pomegranate and kinetin (Kn) in various concentrations and combinations. In the MS medium fortified with 1.5 mg/l BAP, 1.5 mg/l SNP, and 1.5 mg/l Kn, maximum shoot length, shoot per explants, and regeneration % were noted. For root induction, uniform micro shoots were separated and then placed in the rooting media (Murashige and Skoog medium at half strength), supplemented with different ratios of NAA and SNP with 2 mg/L NAA and 0.3 mg/L SNP yielding the highest culture rooting, number of roots, and root length. The described methodology permits the development of multiple pomegranate plants that have been micro propagated. Superoxide dismutase, catalase, and guaiacol peroxidase activities revealed the antioxidant defence mechanism of micro propagated pomegranate micro shoots, which increased shoot development and the number of shoots per explant. Using simple sequence repeat (SSR) markers, the mother plants and proliferating plantlets were checked for genetic integrity; the results showed that all of the plants were the same. The results of this study could be used commercially in the future to produce high-quality pomegranate planting material. |
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https://link.springer.com/article/10.1007/s13369-024-08972-0
